Solid Phase Peptide Synthesis Mechanism

Peptide synthesis is the production of peptide. Over the year different processes and methods were discovered and invented to produce large number of peptides to meet the need of the protein in different areas of medical sciences. The organic chemistry has helped a great deal in peptide synthesis mechanism by which peptides are produced.

Peptide synthesis is robust and fool proof. However, there are certain things which can really disturb the reproducibility of these protocols. Probably the chief amongst all disturbing elements is the quality of DMF. It is incredibly important to use ‘quality’ DMF during the solid phase peptide synthesis to achieve better yield. This means either getting it off the solvent system or opening a new bottle. There are few solid phase peptide synthesis mechanisms that fall under the solid phase peptide synthesis.

The first stage in solid-phase peptide synthesis is the choice; choosing what functional group you want your C -terminus to be:

If you want your C -terminus to be a carboxylic acid use 2-chlorotrityl resin.

If you want your C -terminus to be an amide use Rink amide resin.

If you are making a macrocyclic peptide use 2-chlorotrityl resin.

Once your choice of resin is made you will need to load your first amino acid onto the resin.

1- The process constitutes weighing up of appropriate amount of resin. Generally 300 mg for a 0.1 mmol scale synthesis is used. Unload the resin into a Poly-Prep chromatography column (BioRad).



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